Coding

Part:BBa_I72512

Designed by: Erin Dul   Group: Guests   (2007-06-18)

Enoyl-coa hydratase

Enoyl-coa hydratase, a.k.a. crotonase, based on the open reading frame CAC2712 from C. acetobutylicum ATCC 824 genome. The sequence has been selected because it is part of an operon (butyryl-Coa synthesis operon) that has successfully been cloned into E. coli. It catalyzes the second step in butanol biosythesis, producing crotonyl-CoA from 3-hydroxybutyryl-CoA. It has a His-tag added to its C terminus.

Usage and Biology

<biblio>

  1. Boynton pmid=8655474

</biblio>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 163
    Illegal AgeI site found at 294
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/biofuels
//function/biosynthesis/butanol
Parameters
None